dallaszoqa.blogg.se - Seamless order

To simplify and speed the design process, we provide a free online tool, the GeneArt™ Design Tool for Seamless or High-Order Assembly and Mutagenesis, to help you design your experiment in silico.

The circularized clones obtained from the reaction contain only the sequence of your original vector, inserts, and designated homologies, with no extraneous nucleotide insertions.Ī key step in GeneArt™ Seamless Cloning is the correct design of fragments and oligos with the appropriate homology and spacing to help ensure successful assembly of your clone. Terminal end homology, if needed, is easily incorporated by PCR amplification with custom DNA oligos engineered using our free web tool.Ĭloning Efficiency, Flexibility, and PrecisionĬloning success is independent of the insert sequence and vector type, allowing you to design and add nearly any desired sequence, or combination of sequences, to any plasmid as long as it can be linearized by either restriction enzyme digestion or PCR. The enzyme mix uses a proprietary enzyme/buffer mix to assemble DNA fragments without extra sequences or scars ('seamless'). GeneArt™ Seamless Cloning is a simple, two step process, consisting of in vitro assembly followed by transformation.

Vector Flexibility: use our linear vector or a vector of your choiceįor the cloning of pre-existing DNA elements too long to be amplified by PCR and for final molecules up to 40 Kb, please consider the GeneArt™ Seamless PLUS Cloning and Assembly Kit (1–4 DNA fragments only) and for final molecules larger than 110 Kb, please consider the GeneArt™ High-Order Genetic Assembly System.

Free Tools: design of your final construct and DNA oligos in silico using our free web-based tool that takes you step-by-step through your project.

Speed and Ease: clone DNA fragments, with sequence of your choice, in a single vector (up to 13 kb) no restriction digestion, ligation, or recombination sites required.

Efficiency: pre-cloning option for large fragments for increased cloning efficiency.

GeneArt™ Seamless enzyme mix is the economical choice for creating constructs up to 13 kb with the option for high-throughput assembly. GeneArt™ Seamless Cloning and Assembly Enzyme Mix enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction.